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human regulatory t cell staining kit#3  (Thermo Fisher)


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    Thermo Fisher human regulatory t cell staining kit#3
    Human Regulatory T Cell Staining Kit#3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+regulatory+t+cell+staining+kit/human+regulatory+t+cell+staining+kit/pmc12184707-70-5-15
    Average 90 stars, based on 1 article reviews
    human regulatory t cell staining kit#3 - by Bioz Stars, 2026-09
    90/100 stars

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    Staining:

    Article Title: Long noncoding RNA LIRIL2R modulates FOXP3 levels and suppressive function of human CD4 + regulatory T cells by regulating IL2RA
    Article Snippet: .. Intracellular staining was performed with buffer sets of the Human Regulatory T Cell Staining Kit (eBioscience/ThermoFisher Scientific, Cat# 88-8999-40) by following the manufacturer’s protocol. .. Samples were acquired by BD LSR II or BD LSR Fortessa analyzer (BD Biosciences, Franklin Lakes, NJ) and analyzed either with FlowJo (BD).

    Article Title: Gene Signature of Regulatory T Cells Isolated from Children with Selective IgA Deficiency and Common Variable Immunodeficiency
    Article Snippet: For absolute T reg number evaluation, peripheral blood mononuclear cells (PBMC) from the same person were isolated by the standard Ficoll-Paque (Pharmacia Biotech) density gradient centrifugation. .. Then, PBMCs were stained using the Human Regulatory T cell Staining Kit (eBiosciences, San Diego, CA, USA). ..

    Article Title: Anti-CD3epsilon antibodies
    Article Snippet: .. Various concentrations of test antibodies, positive and negative controls were added and then incubated with the cells for 4.5 hours at 37° C. After incubation, the cells were washed two times with 1% BSA, and then stained for their surface makers by using anti-human CD4-FITC antibody (BD, 550628), and anti-human CD8-PE antibody (BD, 560959), followed by fixation and permeabilization using the Human Regulatory T Cell Staining Kit (eBioscience, 88-8999). .. Post fixation/permeabilization, the cells were detected for cytokine production by using anti-human TNF-APC antibody (BD, 554514) and anti-human IFN-PerCP-Cy5.5 antibody (eBioscience, 45-7319-42).

    Article Title: Boosting anti-tumor immunity with TILT-517 oncolytic adenovirus and checkpoint blockade in renal cell carcinoma
    Article Snippet: 356 For the immune studies related to the syngeneic hamster model, the single-cell suspensions were 357 treated with a 1:1 ratio mix of Human TruStain FcX Receptor Blocking Solution (Biolegend, CA, USA) 358 and Purified Rat Anti-Mouse CD16/CD32 blocking solution (BD Biosciences, NJ, USA), and stained with 359 antibodies targeting CD8+, CD4+, MHC-II+ and CD51+ cells. .. 360 Regulatory T cells presence was analyzed using eBioscience Human Regulatory T Cell Staining Kit 361 (ThermoFisher, MA, USA), according to manufacturer’s instructions. ..

    Article Title: Boosting anti-tumor immunity with TILT-517 oncolytic adenovirus and checkpoint blockade in renal cell carcinoma
    Article Snippet: For the immune studies related to the syngeneic hamster model, the single-cell suspensions were treated with a 1:1 ratio mix of Human TruStain FcX Receptor Blocking Solution (Biolegend, CA, USA) and Purified Rat Anti-Mouse CD16/CD32 blocking solution (BD Biosciences, NJ, USA), and stained with antibodies targeting CD8 + , CD4 + , MHC-II + , and CD51 + cells. .. Regulatory T cells presence was analyzed using eBioscience Human Regulatory T cell Staining Kit (ThermoFisher, MA, USA), according to manufacturer’s instructions. ..

    Article Title: Quantitative genome-scale metabolic modeling of human CD4 + T cell differentiation reveals subset-specific regulation of glycosphingolipid pathways.
    Article Snippet: .. Intracellular stainingwas performed using buffer sets of Human Regulatory T cell Staining Kit (eBioscience/Thermo Fisher Scientific), following the manufacturer’s protocol. .. The following antibodies were used: anti-human FOXP3-PE (eBioscience, Cat. No. 12-4776-42) and rat IgG2a isotype control (eBioscience, Cat. No. 72-4321-77A).

    Incubation:

    Article Title: Anti-CD3epsilon antibodies
    Article Snippet: .. Various concentrations of test antibodies, positive and negative controls were added and then incubated with the cells for 4.5 hours at 37° C. After incubation, the cells were washed two times with 1% BSA, and then stained for their surface makers by using anti-human CD4-FITC antibody (BD, 550628), and anti-human CD8-PE antibody (BD, 560959), followed by fixation and permeabilization using the Human Regulatory T Cell Staining Kit (eBioscience, 88-8999). .. Post fixation/permeabilization, the cells were detected for cytokine production by using anti-human TNF-APC antibody (BD, 554514) and anti-human IFN-PerCP-Cy5.5 antibody (eBioscience, 45-7319-42).



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